Workshop Overview

Recombinant protein purification is a key technique in biotechnology for research, diagnostics, and therapeutic development.

This hands-on workshop will focus on purifying His-tagged Green Fluorescent Protein (GFP) using Immobilized Metal Affinity Chromatography (IMAC).

Participants will learn to:

• Purify GFP protein using IMAC
• Evaluate protein recovery and purity using NanoDrop spectrophotometry
• Measure fluorescence to assess functional protein quality
• Gain practical exposure to essential laboratory techniques in protein biotechnology

Outcome:

Strengthen lab skills and understand a fundamental tool of protein research.

Workshop Overview

Structure-based computational biology is revolutionizing modern drug discovery by allowing researchers to predict, analyze, and optimize biomolecular interactions in silico.

This workshop provides hands-on training in:

• Homology modeling of proteins
• Molecular docking techniques
• Introductory molecular dynamics simulations
• Using AI-driven tools for protein structure prediction and virtual screening

Outcome:

Learn computational methods that accelerate therapeutic research and drug design.

This hands-on workshop covers food processing and quality evaluation instruments:

• Farinograph:
Measures dough mixing behavior by recording resistance during kneading. Determines water absorption, dough development time, and dough stability, helping assess flour quality and gluten strength for bakery applications.

• Gloss Meter:
An optical device that measures surface shine by analyzing reflected light. Used to evaluate appearance and surface quality of chocolates, coatings, fruits, and packaging materials.

• Color Analyzer:
Objectively measures food color using light reflectance and expresses results in L*, a*, b* values. Ensures color uniformity and monitors quality changes during processing.

• Freeze Dryer:
Dehydrates food by sublimation under vacuum after freezing, preserving structure, flavor, and nutrients while extending product shelf life.

Workshop Overview

This session focuses on innovative food manufacturing and sensory evaluation:

3D Food Printing:

• Uses additive manufacturing principles to create customized foods with layer-by-layer deposition of edible materials using CAD designs.
• Enables control over shape, texture, and nutrition.
• Useful for personalized nutrition, product innovation, and sustainable food processing.

Triangle Test:

• A discriminative sensory evaluation method used to identify differences between food samples.
• Three coded samples are presented: two identical, one different. Panelists detect the odd sample based on sensory attributes.
• Widely used for quality control, product comparison, formulation changes, shelf-life studies, and process optimization.
• Results are analyzed statistically for significance.

Outcome:

Learn latest food manufacturing and sensory evaluation methods.

Workshop Overview

High-Performance Liquid Chromatography (HPLC) is an analytical technique used to separate, identify, and quantify components in a mixture.

Key Points:

• Works based on interaction between stationary phase (column) and mobile phase (solvent)
• Widely used in pharmaceutical industry for:
  • Drug analysis
  • Quality control
  • Purity testing
• Helps determine:
  • Drug assay
  • Impurities and degradation products
  • Stability of drugs
• Provides accurate, precise, and reproducible results
• Essential for pharmaceutical manufacturing and research

Outcome:

Learn High-Performance Liquid Chromatography (HPLC) is an analytical technique.

Workshop Overview

SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is a technique used to separate proteins based on molecular weight.

Key Points:

• SDS detergent denatures proteins and gives them a uniform negative charge
• Proteins migrate through a gel under an electric field
• Smaller proteins move faster, larger proteins move slower
• Used in pharmaceutical applications for:
  • Protein purity testing
  • Molecular weight determination
  • Quality control of biopharmaceuticals
• Widely applied in the analysis of vaccines, monoclonal antibodies, and therapeutic proteins

Outcome:

Learn SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) technique.